Epinephrine was determined using a lab-made chemiluminescence (CL) system with air pump. Luminolsodium IO4? chemiluminescence system was employed to produce the luminescence of epinephrine. In the reaction, epinephrine was oxidized to produce superoxide or singlet oxygen by periodate in alkaline solution, which enhanced CL of luminol. For optimization, various buffers, such as phosphate, borate, and tris, were studied in this experiment. Compared to NaOH, the phosphate and borate buffer showed better reproducibility with similar sensitivity. Small amount of sample, 22 レL, was required for a measurement. The limit of quantification for epinephrine was obtained to be ~10?9 g/mL after optimization.
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机译：肾上腺素是使用实验室制造的化学发光（CL）系统和气泵测定的。用发光素IO 4 ？化学发光系统产生肾上腺素的发光。反应中，肾上腺素在碱性溶液中被高碘酸盐氧化生成超氧化物或单重态氧，从而增强了鲁米诺的CL。为了优化，在本实验中研究了各种缓冲液，例如磷酸盐，硼酸盐和Tris。与NaOH相比，磷酸盐和硼酸盐缓冲液的再现性更好，灵敏度相似。测量所需的样品量为22 µL。优化后肾上腺素的定量限为〜10 ？9 g / mL。
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