Microsatellite mapping of genes for branched spike and soft glumes in Triticum monococcum L.

摘要

The locus responsible for branched spike in a branched spike mutant of Triticum monococcum L. (2n = 2x = 14, A(m)A(m) genome) and soft glume in Triticum sinskajae Filat. et Kurkiev (2n = 2x = 14, A(m)A(m) genome) were mapped by genotyping F-2 populations using microsatellite markers. Phenotypic analysis in the cross T. sinskajae PI 418587/a branched spike mutant KT3-24 confirmed that both characters were under control of a recessive allele at a single locus, and they were linked with 26.6 cM. The branched head in T. monococcum (bh(m)) locus was located on chromosome 2A(m)S and the marker Xgwm122 flanked the bh(m) gene distally. Soft glume locus in T. sinskajae was allelic to the soft glume (sog) locus in mm09, a soft glume mutant of T. monococcum. The sog locus was linked with Xwmc644 distally. In the F-2 hybrids of T. monococcum #252/PI 418587 and T. monococcum KT 3-21/PI 418587, sog was linked with Xgwm71. The gene fg which determines a false glume was also located on chromosome 2A(m)S and the recombination between sog and fg (1.6 cM) was obtained in F-2 hybrid of KT 3-21/PI 418587.