Site-selective Cleavage of RNA at Two Sites by Tandem DNAzyme and its Detection by Mass Spectrometry for Genotyping of SNP

摘要

Accurate and low-cost methods of genotyping of single nucleotide polymorphism (SNP) caused by single base-pair substitution are crucially important for prediction of hereditary diseases, design of individualized medicine, and prognosis of SNP-related diseases.1 For example, drug resistance in leukemia is often caused by several point mutations occurred in the leukemogenic gene. Such point mutations that constitute drug-resistant SNPs in chronic myelogenous leukemia (CML) include the T315I mutant, in which threonine (T) at position 315 is replaced with isoleucine (I) due to a single base change (C to U) in the ABL gene.