Multiple Nonenzymatic Labels-Based Impedimetric Aptamer Sensor for the Competitive Detection of Thrombin

摘要

Thrombin (TB) is a coagulation and serine protease that converts soluble fibrinogen to insoluble strands of fibrin as well as catalyzing many other coagulation-related reactions responsible for blood clotting. It plays an important role in thrombosis and haemostasis and its detection is very useful for early diagnosis of intracerebral hemorrhage1 and pulmonary metastasis. It is not present in blood under normal condition but can be elevated at low-micromolar concentrations during coagulation process. Thus, the development of a high sensitive aptamer sensor for the detection of thrombin would be of great interest.