Study on Long-Term Stability of Non-Biofouling Poly[(3-(methacryloylamino)propyl)-dimethyl(3-suIfopropyl)ammonium hydroxide] Films Under Biologically Relevant Conditions

摘要

In the fabrication of biomedical micro- and nano-systems, including cell chips and prosthetic devices, is used intensively polymer grafting, such as surface-initiated polymerization, as an interface controller to preferentially inhibit or promote cell adherence and growth with the molecular-level control For example, we reported that the film of a zwitterionic polymer, poly[(3-(methacryloylamino)propyl)-dimethyl(3-sulfopropyl)ammonium hydroxide] [poly-(MPDSAH)], possessing both anionic sulfonate and cationic quaternary amine, effectively prevented the non-specific adsorption of proteins and adherence of cells. In comparison, a slight structural-variation to poly[(2-(methacryloyl-oxy)ethyl)-dimethyl(3-sulfopropyl)ammonium hydroxide] [poly(MEDSAH)] was found to sustain long-term growth of human embryonic stem cells. It is, however, the long-term film stability that should be considered more seriously for practical use of polymer coating in biomedical devices, besides the intrinsic cell-repelling or cell-attracting property of polymer films; the prolonged desired-functioning of a polymer film is determined absolutely by the long-term stability of the film.