A loop-mediated isothermal amplification method for rapid detection of the multidrug-resistance gene cfr

摘要

We developed and evaluated the specificity and sensitivity of a loop-mediated isothermal amplification (LAMP) method for rapid detection of the multidrug-resistance gene . cfr. A pair of outer primers and a pair of inner primers and one loop primer were specially designed for recognizing seven distinct sequences on the target . cfr gene. The amplification reaction was performed within only 35. min under isothermal conditions at 63. °C in a regular water bath with visual measurement. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of 1. pg per tube of chicken . Staphylococcus sciuri genomic DNA. The detection rate of . cfr gene for 50 Staphylococcus clinical strains by LAMP assays was 16% and appeared 100% consistence with the result by PCR method. The LAMP method reported here demonstrated a potential and valuable means for detection of the multidrug-resistance gene . cfr: easy, rapid, visual, specific, accurate and sensitive, especially useful for on-the-spot investigation.