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COMPOSITION AND METHODS FOR RAPID DETECTION OF HIV BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)
COMPOSITION AND METHODS FOR RAPID DETECTION OF HIV BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)
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机译:通过环介导的等温扩增(LAMP)快速检测HIV的成分和方法
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摘要
Methods and compositions for detection of HIV nucleic acids in a sample, such as a biological sample obtained from a human subject, are provided according to embodiments of the present invention which include providing a reaction mixture including at least one LAMP, accelerated LAMP, RT-LAMP or RT-accelerated LAMP assay primer set specific for HIV-I or HIV-2 nucleic acids and the biological sample to be tested for presence of HIV-I and/or HIV-2 nucleic acids; incubating the reaction mixture under conditions suitable to produce a LAMP assay reaction product; and detecting the reaction product. Primers and primer sets for use in LAMP assays of HIV-I or HIV-2 nucleic acids are provided.
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