Rapid discrimination of the common species of the stored product pest Liposcelis (Psocoptera: Liposcelididae) from China and the Czech Republic, based on PCR-RFLP analysis.

摘要

Psocids of the genus Liposcelis (Psocoptera: Liposcelididae) are stored product pests that are difficult to identify morphologically. A molecular method based on Restriction Fragment Length Polymorphism (RFLP) of the PCR-amplified 16S rDNA gene was developed for the rapid discrimination of four common species (L. bostrychophila, L. entomophila, L. decolor, and L. paeta). Different developmental stages and populations (P.R. China and Czech Republic) were tested. One DNA fragment of about 500 bp in length was amplified from genomic DNA and the fragment was then digested using the restriction endonuclease DraI. Identification of the relevant banding pattern allowed all the developmental stages and both sexes to be discriminated in the species tested. The banding patterns of L. entomophila from all populations were identical, while the relevant restriction digests and sequence analysis confirmed that the Chinese and Czech populations of L. bostrychophila, L. decolor, and L. paeta differed. In conclusion, PCR-RFLP with one pair of primers (16Sar and 16Sbr) and one restrictive endonuclease, DraI, proved a reliable method for rapidly discriminating the Liposcelis species tested.