Differentiation of raw or processed eel by PCR-based techniques: restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP)

摘要

PCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b gene was amplified. The amplicon was sequenced and restriction enzymes were selected for RFLP analysis; a short part (123 bp) of the 464 bp section was amplified by another set of primers and used for SSCP analysis.The suitability of RFLP and SSCP for differentiating hot-smoked eel was verified by a collaborative study.