Response of Turkish Melon Genotypes to Fusarium oxysporum f. sp. melonfe Race i Determined by Inoculation Tests and RAPD Markers

摘要

The response to Fusarium oxysporum f. sp. melonis (F.o.m.) race 1 of 79 Cucumis melo L. genotypes - 56 accessions collected from different parts of Turkey, 5 local cultivars and 18 foreign genotypes - was determined by using pathogenicity tests and RAPD markers (E07 and G17). For pathogenicity tests, at least 15 individuals of each melon genotype were artificially inoculated by either conidia suspension culture or sand culture of F.o.m. race 1. Melon seedlings were evaluated on a 0-3 scale for the presence of vascular browning two weeks after inoculation with conidia suspension culture resp. four weeks after inoculation with sand culture. The disease incidence on the 79 melon genotypes ranged from 0 to 100 %. Seven melon genotypes were resistant (CU129, CU258, T8, Y9, Y10, Y15, and Y63), three melon genotypes (CU280, CU309, and Tl) were heterogeneous, and all the others were susceptible to F.o.m. race 1 based on disease incidence and vascular browning. The success of the E07 marker in detecting susceptible genotypes was higher than that of G17; the mistmatch ratios of E07 and G17 markers were 5.06 and 58.23 %, respectively.