No evidence for enhanced parvalbumin concentration in light muscle of transgenic coho salmon (Oncorhynchus kisutch)

摘要

The expression of parvalbumin, a major allergenic protein of fish muscle, was determined in rapidly growing transgenic coho salmon (Oncorhynchus mykiss) containing the growth hormone (GH) gene construct OnMTGH1. Three different methods for parvalbumin analysis were used: (1) measurement of the mRNA concentration by real-time RT-PCR; (2) isoelectric focusing of sarcoplasmic proteins; (3) assessment of amount of heat-stable, Ca2+-binding sarcoplasmic proteins by measuring absorbance spectra of "stains-all"aEuro"protein complexes. Compared to non-transgenic coho salmon, no indication for enhanced expression of parvalbumin in transgenic fish was found either at the mRNA or at the protein level.