Development and characterization of SSR markers in Chinese cherry (Prunus pseudocerasus Lindl.).

摘要

24 simple sequence repeat (SSR) markers were isolated and characterized in P. pseudocerasus Lindl. from the cultivar 'Laiyang Short cherry'. To amplify SSR loci primers were developed to flank each repeat region, and they were initially tested on 6 cherry samples using chemiluminescence detection. 18 loci where shown to be polymorphic and the number of alleles detected per locus varied from 2 to 6.12 loci were chosen for the analysis of 26 cultivars grown in North China using the semi-automatic system ABI PRISM 377. These 12 markers showed a high level of genetic polymorphism with a total of 75 alleles; the number of alleles ranged from 4 to 10 per locus, with an average level of 6.3. The mean expected and observed heterozygosity was 0.743 (range: 0.680-0.845) and 0.829 (range: 0.730-0.930) respectively. The estimated frequency of null alleles showed a positive value for 3 loci, but except for 1 locus, the values were very low. The total value for the probability of identity was 7.01x10-13. Paternity exclusion probability was very high (0.999), sufficiently high to study pollen flow.