Production of Extracellular Protease by a Brazilian Strain of Beauveria bassiana Reactivated on Coffee Berry Borer, Hypothenemus harnpei

摘要

Studies were carried out on extracellular protease production by Beauveria bassiana CG432 in liquid mediumcontaining glucose and yeast extract. B. Bassiana presented active growth after lag period of 24 h., produced 80%of the total of the extracellular protease activity in 48 h which was maximum on the 5th culture day. Theextracellular protease presented optimum activity at 60 deg C, was stable up to 1M Cl~-, maintained the stability during15 day at 4 deg C and -18 deg C, but was not stable if frozen repeatedly.