Aspergillus niveus Blochwitz 4128URM: new source for inulinase production.

摘要

The production of extracellular inulinase by Aspergillus niveus 4128URM isolated from sunflower rhizosphere, and the properties of the enzyme were investigated. Inulinase, which was produced by A. niveus in a culture medium containing inulin as substrate, was dependent on inulin concentration and fermentation time. The maximum inulinase activity was significantly superior at inulin concentrations of 15 and 20 g/litre at 72 h of incubation. However, the best inulin concentration for inulinase production was 20 g/litre after 72 h of incubation. The optimum pH for inulinase production were 4.0 and 4.8, and the optimum temperature was 45 degrees C. Using Sephadex G-75 chromatography, inulinase was purified 34.65-fold, yielding 53.63%. It is suggested that A. niveus 4128URM could be a promising strain for inulinase production in food industries..