L-Carnitine transport in mouse renal and intestinal brush-border and basolateral membrane vesicles

摘要

We characterized the uptake of carnitine in brush-border membrane (BBM) and basolateral membrane (BLM) vesicles, isolated from mouse kidney and intestine. In kidney, carnitine uptake was Na~+-dependent, showed a definite overshoot and was saturable for both membranes, but for intestine, it was Na~+-dependent only in BLM. The uptake was temperature-dependent in BLM of both kidney and intestine. The BM transporter in kidney had a high affinity for carnitine: apparent K_m = 18.7 μM; V_(max) = 7.85 pmol/mg protein/s. In kidney BLM, similar characteristics were obtained: apparent K_m = 11.5 μM and V_(max) = 3.76 pmol/mg protein/s. The carnitine uptake by both membranes was not affected within the physiological pH 6.5-8.5 Tetraethylammonium, verapamil, valproate and pyrilamine significantly inhibited the carnitine uptake by BBM but not by BLM. By Western blot analysis, the OCTN2 (a Na~+-dependent high-affinity carnitine transporter) was localized in the kidney BBM, and not in BLM. Strong OCTN2 expression was observed in kidney and skeletal muscle, with no expression in intestine in accordance with our functional study. We conclude that different polarized carnitine transporters exist in kidney BBM and BLM. L-Carnitine uptake by mouse renal BBM vesicles involves a carrier-mediated system that is Na~+-dependent and is inhibited significantly by specific drugs. The BBM transporter is likely to be OCTN2 as indicated by a strong reactivity with the anti-OCTN2 polyclonal antibody.