EXPRESSION OF A BEE-VENOM PHOSPHOLIPASE A_2 FROM APIS CERANA CERANA IN ESCHERICHIA COLI

摘要

The venomous phospholipase A2 (AcPLA_2) coding reading region of the Chinese honeybee (Apis cerana cerana), which is composed of 405 bp encoding a mature glycosylated peptide with 134 amino residues was transformed into the expression vector pETblue-1. Then the recombinant vector was introduced into Escherichia coli Tuner (DE3) plac I for expression. Analysis result of SDS-PAGE showed that the expression products had a protein band of about 15 kD. Detection of western blot using ant-European honeybee(Apis mellifera) phospholipase A_2 (AmPLA_2) polyclonal serum as the first antibody showed that the expression products appeared a special blot same as the native AmPLA_2. The result demonstrated that the AcPLA_2 peptide had been expressed in E. coli and the AcPLA_2 has the similar antigenicity as the AmPLA_2.