Diet of Japanese eels Anguilla japonica in the Kojima Bay-Asahi River system, Japan

摘要

The diet of Japanese eels, Anguilla japonica, was investigated using stomach content and stable isotope analyses. Stable isotope enrichment of carbon and nitrogen (Delta delta C-13 and Delta delta N-15) was first estimated for A. japonica by comparing the isotopic signatures (delta C-13 and delta N-15) of reared eels to that of their food. The estimated isotope enrichment was then applied to the diet estimation of A. japonica in the Kojima Bay-Asahi River system, Japan, combined with conventional stomach content analysis. Stable isotope enrichment varied among tissues, from 0.2aEuro degrees to 0.8aEuro degrees for carbon and from 1.3aEuro degrees to 2.1aEuro degrees for nitrogen. Nitrogen isotope enrichment of A. japonica muscle estimated in this study was 2.1aEuro degrees, which was different from the previously reported mean delta N-15 enrichment of several animals of 3.4aEuro degrees. These results indicate that isotope-based diet estimations for A. japonica need to use species- and tissue-specific values of isotope enrichment. In the diet analysis, stomach contents and stable isotopes revealed that (1) A. japonica appear to usually feed on a single type of prey species in each feeding session, (2) principal prey species were mud shrimp, Upogebia major, in brackish Kojima Bay and crayfish, Procambarus clarkia, in the Asahi River, (3) A. japonica in Kojima Bay primarily depend on the pelagic food web as a carbon source due to mud shrimp being filter feeders and eels in the Asahi River primarily depend on the littoral food web. Based on these results and the recently reported eel movements between Kojima Bay and the Asahi River, it appears that A. japonica can adapt to various feeding environments as opportunists, but also utilize the food resources by targeting a single type of prey species during a single feeding session.