Independent laboratory method validation for the determination of myclobutanil residues in cucumber

摘要

A simple and inexpensive method was developed using solid-phase extraction extraction, together with high performance liquid chromatographic method with UV detection for determination of Myclobutanil residues in cucumber. The evaluated parameters include the extracts by silica gel sorbent using Ethyl acetate and acetonitrile solvents. The method was validated using cucumber samples spiked with Myclobutanil at different fortification levels (0.01 and 0.1 mg/kg). Average recoveries (using each concentration six replicates) ranged 86-94%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-5.0 ug/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.003 mg/kg and 0.01 mg/kg respectively. Finally the cucumber samples were re analyzed by HPLC.