Location of the toxic molecule abietic acid in model membranes by MAS–NMR

摘要

Abietic acid, the major component of conifer oleoresin, is an environmental toxic molecule with potential hazard to animal and plant life. Being amphipatic, the study of its location and the interaction with membrane components is important to get insight into the mechanism of its toxic action. High resolution magic angle spinning natural abundance 13C nuclear magnetic resonance studies have been undertaken in order to assess its location in egg yolk phosphatidylcholine multilamellar vesicles model membranes. 13C spin-lattice relaxation times in the presence of Gd3+, a paramagnetic agent, of both the phospholipid and abietic acid molecules have been measured in order to obtain information on molecular distances (see J. Villalaín, Eur. J. Biochem. 241 (1996) 586–593). The molecule of abietic acid is placed in the upper part of the palisade structure of the membrane, its carboxyl group is in close proximity to the phospholipid ester groups and it does not extend beyond the C4/C7 carbons of the phospholipid molecule.