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Potentiometric coulometry based on charge accumulation with a peroxidase/osmium polymer-immobilized electrode for sensitive determination of hydrogen peroxide

机译:基于过氧化物酶/ os聚合物固定电极电荷积累的电位库仑法,用于过氧化氢的灵敏测定

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摘要

The charge accumulation due to peroxidase (POD)-catalyzed reduction of H_2O_2 in a test solution (4 μL) by Os(II) in a POD/PVI[Os(dmebpy)_2Cl]-immobilized layer on an electrode (PVI = poly(1-vinylimidazole), dmebpy = 4,4′-dimethyl-2,2′-bipyridine) was monitored potentiometrically for the detection of H_2O_2. Before potentiometry, the Os(II)/Os(III) ratio of the modified electrode was controlled by pre-electrolysis at a given potential in a separated electrolysis cell. The redox potential of the Os polymer film in the test solution shifted to the positive side on the addition of H_2O_2 and reached a constant value due to the accumulation of Os(III) in the film. The total amount of the accumulated charge was determined from the area of the portion corresponding to the redox potential shift on a reversible cyclic voltammogram recorded separately. The low detection limit (5 pmol H_2O_2) was realized with 82-90% of the recovery percentage.
机译:过氧化物酶(POD)催化Os(II)在电极上POD / PVI [Os(dmebpy)_2Cl]固定层中的Os(II)还原测试溶液(4μL)中H_2O_2引起的电荷积累(PVI =聚(用电位计监测1-乙烯基咪唑(dmebpy = 4,4′-二甲基-2,2′-联吡啶)以检测H_2O_2。在电位计之前,通过在分离的电解池中以给定电势进行预电解来控制修饰电极的Os(II)/ Os(III)比。加入H_2O_2后,测试溶液中Os聚合物薄膜的氧化还原电势向正侧移动,由于薄膜中Os(III)的积累,氧化还原电势达到恒定值。根据在单独记录的可逆循环伏安图上对应于氧化还原电势偏移的部分的面积来确定累积电荷的总量。实现了低检测限(5 pmol H_2O_2),回收率达到82-90%。

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