Development of Amperometric Glucose Biosensor Based on Reconstitution of Glucose Oxidase on Polymeric 3-Aminophenyl Boronic Acid Monolayer

摘要

An amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and subsequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is described. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction and oxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at +0.5 V with a high sensitivity (5.94 mA/mM) and relatively good response time (~12 s) in a wide concentration range of 1-17 mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.