Molecular evidence of phytoplasmas in winter oilseed rape, tomato and corn seedlings

摘要

To evaluate the seed transmission of phytoplasmas in three herbaceous hosts material from different geographical origin but all collected from infected or symptomatic mother plants was used. Almost 1,000 seeds were germinated under controlled conditions and 652 seedlings were obtained. Samples were grouped in 214 samples and 74 of them resulted positive to phytoplasma presence after three to 90 days from germination by nested PCR assays on 16S ribosomal gene. All the tested species i.e. winter oilseed rape, tomato and corn resulted carrying phytoplasmas belonging to the ribosomal groups retrieved in the infected mother plants.