Effects of endogenous regulators of endothelial no synthase on nitric oxide homeostasis and blood serum lipoproteins during experimental diabetes mellitus

摘要

Experimental diabetes mellitus was associated with the development of oxidative stress and a decrease in blood concentration of total nitric oxide (NO) metabolites. Administration of L-arginine induced positive changes in the LPO-antioxidant enzyme system and elevated NO concentration in blood serum, whereas L-NAME, inhibitor of eNOS (NOS-III) increased LPO intensity via SOD inhibition and reduced NO content. Combined administration of Q10 and L-arginine led the suppression of oxidative stress and significant increase in NO level. Combined treatment with Q10 and L-NAME partly abolished the effects of the inhibitor on the parameters of the LPO-antioxidant enzyme system and NO concentration. In all variants of the study, Q10 stimulated eNOS expression and increases NO bioavailability by reducing the levels of total cholesterol and LDL and increasing HDL content in blood serum.