Assessment of genetic variation in tomato (Solanum lycopersicum L.) genotypes using SSR molecular markers

摘要

The present investigation regarding study of genetic variation of 19 genotypes of tomato (Solatium lycopersicum L.) started with the screening of polymorphic microsatellite markers. From the identified 11 polymorphic SSR markers, a total of 261 polymorphic amplified fragments were obtained. The average polymorphism information content (PIC) was 0.990 ranging from 0.979 (SSR-110) to 0.995 (SSR-253). Two hundred sixty one SSR loci were used to calculate Jaccard's distance coefficients for UPGMA clusteranalysis. The generated dendrogram of 19 tomato genotypes showed low level of similarity among these genotypes. Two major clusters were generated at 43% level of similarity. The cluster A consists of two genotypes whereas cluster B consist of rest of 17genotypes. The results also showeds that the genotypes Arka Vikas and 2012TODVAR-2 were 100% similar. Therefore, these markers may be used for wide range of practical application including varietal identification and pre-screening for distinctiveness oftomato genotypes.