Expression of P311, a transforming growth factor beta latency-associated protein-binding protein, in human kidneys with IgA nephropathy.

摘要

BACKGROUND: In cultured NIH3T3 cells, P311 binds to the transforming growth factor-beta (TGF-beta)-1 latency-associated protein (LAP) and induces the myofibroblast phenotype. In this study, we determined the levels of P311 and TGF-beta1 proteins in tubulointerstitial tissue of patients with different severities of immunoglobulin-A nephropathy (IgAN), and analyzed the relationships between P311 protein expression and clinical data. METHODS: A total of 57 patients with IgAN and 5 controls (from partial nephrectomy) were included. P311 and TGF-beta1 protein expression were measured by immunohistochemistry and clinical data (proteinuria, serum creatinine (Scr), eGFR and biopsy index) were recorded. The relationship between P311, TGF-beta1, and clinical data was analyzed. RESULTS: P311 expression was significantly higher in the kidneys of IgAN patients than in controls and was higher in patients with advanced pathological grades of IgAN. P311 protein expression in tubulointerstitial tissue correlated with TGF-beta1 and proteinuria. P311 expression was higher in patients with Scr > 133 mumol/L than in patients with Scr < 133 mumol/L. CONCLUSION: P311 protein expression in the kidneys of IgAN patients correlates with TGF-beta1 expression and with proteinuria. P311 might be a key cytokine in renal fibrosis and be involved in the progression of IgAN.