Protection of Salvia Miltiorrhiza against Acute Sodium Nitrite Poisoning in Mice

摘要

Objective: The study was evaluated the protection of salvia miltiorrhiza injection against acute sodium nitrite poisoning in mice. Materials and methods: Forty age-matched male C57mice were randomly divided into control group, sodium nitrite poisoning group (SN), Methylene blue (MB) and Salvia miltiorrhizae-treated(SM) group. In SN, MB and SM group, 200 mg/kg sodium nitrite was injected intraperitoneal. Mice in MB and SM group were given 1.5 mg/kg Methylene blue and 0.2 ml Salvia miltiorrhiza injection immediately after sodium nitrite injection respectively. All mice were sacrificed after 24 h of sodium nitrite injection. Blood were collected for the measurement of methemoglobin, serum were isolated for the measurement of TNFa, IL-6 and IL-10 concentration by ELISA. HE staining and TUNEL staining were applied for tissue pathological changes and apoptosis detection. Results: Mice in SN and SM group demonstrated more higher blood methemoglobin and Serum TNFa and IL-6 but lower IL-10 levels than control group (P < 0.05). HE staining and TUNEL staining results showed that all mice given sodium nitrite developed histological damage accompanied with increased apoptotic cell number in heart, lung, liver and kidney tissue. But the values were much less severe in MB and SM group than those in SN group(P < 0.05). There is no statistical difference between MB and SM group. Conclusion: Salvia miltiorrhizae injection reduced tissue injury induced by Sodium nitrite effectively, which may served as a beneficial treatment accompanied with methylene blue to rescue acute sodium nitrite poisoning patients.