Determination Of Sodium Salvia Miltiorrhiza's Optimal Analytical Conditions Detected By HPLC Method

摘要

The optimal analytical conditions of sodium salvia miltiorrhiza are detected by HPLC methods. SinoChrom ODS-BP C18 is selected as chromatographic column. The optimal experimental conditions under 280 nm wavelength UV detector include the column temperature: 28°C, ratio of methanol/water: 25/75 (v/v), and flow rate: 1.0 ml/mol. The correlation coefficient is 0.9983, and RSD is 1.4%, when the linear range is 0.15～1.8μg/μL. In the experiment, the method is verified to be simple and reliable, and which can be used for the quality control in process of salvia miltiorrhiza extraction and purification.