Production of Secondary nematicidal metabolites from Purpureocillium lilacinum using Karanja Deoiled Cake

摘要

Karanja (Pongamia pinnata) deoiled cake was evaluated as an alternative substrate for the production of major nematicidal metabolite, leucinostatins, from Purpureocillium lilacinum 6029. Comparison between the chromatograms confirmed the enhanced production of leucinostatins in Karanja deoiled cake medium over commercially available Czapeck's Dox medium. Nematicidal activity assays showed that the fungal filtrate from Karanja deoiled cake medium was toxic (LT -1.46 h) killing 100 % Meloidogyne incognita larvae in 12h. Our findings will help designing potent bionematicidal formulation comprising ofbiocontrol agent (P. lilacinum) and biodiesel by-product (Karanja deoiled cake) together in an environment-compatible approach.