CYTOTOXICITY OF LIVE WHOLE CELL, HEAT KILLED CELL AND CELL FREE EXTRACT OF LACTOBACILLUS STRAIN IN U-87 HUMAN GLIOBLASTOMA CELL LINE AND MCF-7 BREAST CANCER CELL LINE

摘要

In this experiment we have performed a comparative study of Live Whole Cell (LWC), Heat Killed Cell (HKC) and Cell Free Extract (CFE) of Lactobacillus plantarum KP894100 and Lactobacillus acidophilus KP942831 on the 2 different human cell line U-87 (Glioblastoma cell line) and MCF-7 (Breast cancer cell line). Cell viability was determined by the 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cell lines were seeded in the growth medium supplemented with 10% fetal bovine serum and lOOfigl ml penicillin, streptomycin and cultured in a humidified 5% C02 incubator at 37C. Cell densities were maintained 5 x 10s viable cells/ml. Different concentration of LWC, HK cells and CFE (300fig 600 fig, 900 fig 1200 fig 2400 fig 2700 figI ml) were added to the cancer cells for 24 h. The CFE of Lactobacillus acidophilus KP942831 was found to inhibit the growth of MCF-7 cell in a dose-dependent manner as detected by the MTT assay, suggesting that these strains might play an important role in cancer treatment. According to our results, the CFE of Lactobacillus plantarum has stronger cytotoxicity effects comparable to those of CFE, HKC and LWC of LA.