Augmentation of anti-tumour activity of cisplatin by pectin nano-conjugates in B-16 mouse model: pharmacokinetics and in-vivo biodistribution of radio-labelled, hydrophilic nano-conjugates

摘要

Nanoconjugates have matured from simple devices to multifunctional, biodegradable, non-toxic and non-immunogenic constructs, capable of delivering synergistically functioning drugs in vivo. The present study evaluates the efficacy of drug polymer self folding nano-conjugates of pectin-cisplatin to enhance blood circulating levels of cisplatin. Physical characterisation was done by DLS, zeta potential and TEM. Pharmacokinetics and bio-distribution of the ~(99m)Tc labelled pectin-cisplatin nano-conjugates and cisplatin per se was performed at various time points in normal and tumour bearing C57B16 mice, and Gamma Scintigraphic imaging done in rabbits. Nano-conjugates showed prolonged plasma residence and increased t_(1/2) of cisplatin ～11.26 h. Altered bio-distribution was observed with nano-conjugates, as negligible accumulation of cisplatin was observed in kidney and has the potential to reduce nephrotoxicity. We preliminarily investigated the chemotherapeutic potential of pectin-cisplatin nanoconjugates, cisplatin and pectin on murine melanoma B16 cells in vitro and in-vivo. Mouse B16F10 melanoma cells were cultured in vitro in DMEM media containing 10% FBS, nonessential amino acids and antibiotics in a 5% CO_2 incubator at 37℃ and the effect of pectin, cisplatin singly and in combination i.e., pectin-cisplatin conjugate was assessed by MTT assay. In vivo studies were performed by solid tumour models viz., B16F10 on C57B16 mice. Antitumour efficacy was monitored by measuring tumour burden. Combination therapy led to significantly delayed tumour growth and improved survival in vivo. Tumour regression studies clearly indicate that pectin augments the activity of cisplatin as a three-fold reduction in tumour volume was observed.