Beta2-adrenergic receptor genotype influences the effect of nonselective vs. selective beta-blockade on baroreflex function in chronic heart failure.

摘要

Genomic DNA was isolated from peripheral blood using an Auto-pureLS apparatus (Gentra Systems, Minneapolis, MI, USA). p2AR genotypes at the alleles encoding for positions 16 and 27 were determined by the big-dye terminator sequencing technique, for which a pair of primers was designed (using Primer3; http://frodo.wi. mit.edu/primer3/). The sequence reactions were performed using fluorescently labeled dideoxy chain terminations with a Big Dye Terminator ABI Prism kit (Applied Biosystems, Foster City, CA, USA), analyzed (GeneCodes Co, Ann Arbor, Ml, USA) on an Applied Biosystems automated DNA sequencer (model 3730).