Purification and Characterization of Asparaginase from Solid State Culture of Aspergillus Niger AK10

摘要

An asparaginase producing fungus, Aspergillus niger strain AK10, was isolated from raw material for animal feed production. The enzyme production was done using solid state fermentation on the medium containing soybean meal and water at 1:1 ratio. The4-day-old culture was extracted, purified and determined for the general properties. From SDS-PAGE, it was 91.4 kDa in size. The enzyme functioned maximally at pH 8.6 and 37°C and was most stable when stored at pH 8.0-8.6 and 40°C - 50°C. AK10 was slightly sensitive to (NH_4)2SO_4 and was insensitive to NaCl and monosodium glutamate. The potential application of AK10 asparaginase in reducing asparagine content in blended potato has been demonstrated.