首页> 外文期刊>International Journal of Biological Macromolecules: Structure, Function and Interactions >Long-size isoelectricfocusing (IEF) in flexible silicone tubes: an application to semi-preparative fractionation of proteins
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Long-size isoelectricfocusing (IEF) in flexible silicone tubes: an application to semi-preparative fractionation of proteins

机译:柔性硅胶管中的长尺寸等电聚焦(IEF):在蛋白质半制备分离中的应用

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摘要

Soluble proteins extracted from porcine brains were subjected to a series of optional fractionation steps on various chromatographic media including a novel device for semi-preparative isoelectrofocusing (IEF) carried in a flexible silicone tube. The dimensions of the IEF granulated gel beds can be varied from 40 to 75 cm (length) and 0.4-1.6 cm (diameter) which are dependent on the protein's concentration. An average optimal focalisation time of proteins is dependent of the tube length, its diameter and complexity of proteins' mixtures but it is usually reached during 15,000-30,000 V h. A series of sequential protein's fractionation techniques including semi-preparative IEF carried in the flexible silicone tube with the following dimensions: 75 cm in length and 1.6 cm in diameter permitted for observation and partial characterisation of several proteins whose expression levels are specifically high in the brain. (C) 2004 Elsevier B.V. All rights reserved.
机译:从猪脑中提取的可溶性蛋白在各种色谱介质上进行一系列可选的分离步骤,包括在柔性硅胶管中搭载的新型半制备等电聚焦(IEF)装置。 IEF颗粒凝胶床的尺寸可以在40到75厘米(长度)和0.4-1.6厘米(直径)之间变化,这取决于蛋白质的浓度。蛋白质的平均最佳聚焦时间取决于试管的长度,其直径和蛋白质混合物的复杂性,但通常可以在15,000-30,000 V h内达到。一系列顺序蛋白质的分离技术,包括在挠性硅胶管中携带的具有以下尺寸的半制备型IEF:长75 cm,直径1.6 cm允许观察和部分表征大脑中表达水平特别高的几种蛋白质。 (C)2004 Elsevier B.V.保留所有权利。

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