Expression of bovine beta-lactoglobulin as a fusion protein in Escherichia coli: a tool for investigating how structure affects function

摘要

A synthetic gene for bovine beta-lactoglobulin-variant-A (beta-LgA), designed to incorporate a number of restriction sites to form suitable "cassettes" for future site-directed mutagenesis experiments, was constructed as three separate fragments, F1, F2 and F3. F1 was made using the shotgun approach while F2 and F3 were synthesised using PCR.