Optimization of heavy metal-containing dye Acid Black 172 decolorization by Pseudomonas sp. DY1 using statistical designs.

摘要

Based on the potential decolorization ability of Pseudomonas sp. strain DY1, a Plackett-Burman design was used to select the operational parameters for decolorization of heavy metal-containing dye Acid Black 172. Four significant parameters, including pH, temperature, concentrations of Fe3+ and NaH₂PO₄, were optimized. A quadratic model obtained from the response surface design was constructed on experimental data. The optimal condition for decolorization was found to be pH 6.23, 30 degrees C, 8.0 mM of Fe3+, and 10.0 g/L of NaH₂PO₄. The confirmatory experiments (86.0% decolorization percentage within the confidence interval) subsequently verified the accuracy of the experimental model. Moreover, the decolorization under the optimal condition fitted the logarithmic model well (R2=0.964). This strain really offers an alternative for bioremediation of soil or water contaminated by heavy metal-containing dye.