Results of influenza virus detection using different methods

摘要

The timely and reliable laboratory evidences are vital factors for field epidemiologists trying to control outbreaks of infectious diseases and for the practicing clinicians to properly manage disease cases. Therefore, analysis of new detection methods in comparison to the routine "classical" methods is essential to select new methods to be introduced into health service practices, especially in developing countries. In this study we have compared rt-RT-PCR detection of influenza viruses and direct fluorescent-antibody assay using R-Mix hybrid cells (A549&MvlLu) with the "classical" cell culture methods in developing country settings. Materials and methods Clinical samples In this study, we analyzed 503 nasopharyngeal swabs collected during 2008-2009 influenza season from ILI patients of the Baganuur district of Ulaanbaatar and Selenge province, Mongolia, and 7000 nasopharyngeal swabs collected in 133 ISSSs during 2009-2010 influenza season.