Insecticidal activity of native Bacillus thurlnglensisisolates against Diamond back moth (DBM), Plutella xylostellaon cabbage

摘要

Bioefficacy of 100 native Bacillus thuringiensis isolates were evaluated. Isolates DBT-772 andDBT-338 recorded 100per cent mortality against 3rd instar larvae o/Plutella xylostella and molecular characterization oflepidopteran specific cryl, cry2, cry8, cry9 and cry 20 genes reviled that cryl as the dominant gene (54.7%) and none of the eleven isolates recorded the presence of cry 20 gene. Consortium of four isolates DBT-787, DBT-338, DBT-772 and DBT-2370 recorded synergistic effect on DBM larvae.