Bactericidal activity of manganese (IV) complex of 2-methylamino-pyridine against Streptococcus pyogenes and Staphylococcus aureus

摘要

Objective: To investigate the bactericidal activity of manganese (IV) complex of 2-methylamino-pyridine against Streptococcus pyogenes (5. pyogenes) and Staphylococcus aureus (S. aureus). Materials and Methods: The inhibitory effect of the complex was studied on the molecular level and by turbidity measurement, Treatment of bacteria was carried out using 5, 10, 25, 50 and 100 umol of the complex per ml of culture media. Results: The results showed that the growth of S. pyogenes rapidly decreased with increasing concentrations of the complex. In contrast, the complex caused no significant decrease in the growth rate of 5. aureus. The molecular level studies showed that four protein bands, with apparent molecular weights of 19, 2.3, 30 and 54 Kda, respectively, increased in the protein pattern of the .5. pyogenes extract after the complex treatment using silver stained polyacrylamide gels, under reducing condition, However, there was no detectable change in the protein pattern of the S. aureus extract after the complex treatment. No DNA damage was detected while using agarose gel electrophoresis and ethidium bromide staining in both types of bacteria. Conclusion: Manganese (IV) complex of 2-methylamino-pyridine showed an apparent antibacterial inhibitory effect against S. pyogenes, but 5. aureus was apparently resistant.