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首页> 外文期刊>Indian Journal of Experimental Biology >Comparative expression of two abscisic acid-inducible genes and proteins in seeds of aromatic indica rice cultivar with that of non-aromatic indica rice cultivars
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Comparative expression of two abscisic acid-inducible genes and proteins in seeds of aromatic indica rice cultivar with that of non-aromatic indica rice cultivars

机译:两种in酸诱导基因和蛋白质在芳香in稻品种种子和非芳香in稻品种种子中的比较表达

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摘要

As an integral part of stress signal transduction, the phytohormone abscisic acid (ABA) regulates important cellular reactions, including up-regulation of stress-associated genes, the products of which are involved directly or indirectly in plant protection. Being accompanied by an increased endogenous ABA level, the matured seeds, embryo and aleurone tissues of cereals accumulate several genes and proteins, associated with desiccation. The present study was aimed at investigating how the contrasting rice genotypes, varying in their salt-stress sensitivity, differ with respect to the expression pattern of two abiotic stress-inducible genes, Rab16A and SamDC, and corresponding proteins, in the seeds, at the background level (dry or water-imbibed state) and ABA-imbibed conditions, which could be related to the varietal differences in tolerance. The rice genotypes selected were M-1-48 (salt-sensitive), Nonabokra (salt-tolerant) and Gobindobhog (aromatic). An extremely low abundance of Rab16A or practically undetectable SamDC transcripts were observed in M-1-48 and Gobindobhog seeds under control conditions, induced only after exogenous ABA treatment, whereas they were expressed at a much higher level even in dry and water-imbibed seeds of Nonabokra, and lesser induced by ABA. The RAB16A (= dehydrin) and SAMDC protein expression in the three varieties were also identical to the gene expression patterns. Thus, the expression was stress-inducible in M-1-48 and Gobindobhog, while constitutive in Nonabokra. Our study reflected the similarity of the molecular responses to exogenous ABA of the seeds of the aromatic rice Gobindobhog to that of the salt-sensitive M-1-48, in exhibiting lower expression of stress-tolerant proteins only after stress. This work also proved that variation in gene/protein expression in seeds could be highly correlated with the variation in the tolerance mechanism of rice varieties.
机译:作为胁迫信号转导的组成部分,植物激素脱落酸(ABA)调节重要的细胞反应,包括胁迫相关基因的上调,这些基因的产物直接或间接参与植物保护。伴随着内源性ABA水平升高,谷物的成熟种子,胚胎和糊粉组织积累了与干燥相关的几种基因和蛋白质。本研究旨在调查在盐胁迫敏感性下变化的不同水稻基因型在种子上的两个非生物胁迫诱导基因Rab16A和SamDC以及相应蛋白质的表达模式方面如何不同。本底水平(干旱或水分吸收状态)和ABA吸收条件,可能与耐受性的品种差异有关。选择的水稻基因型为M-1-48(对盐敏感),Nonabokra(对盐敏感)和Gobindobhog(对芳香族)。在对照条件下,仅在外源ABA处理后诱导的M-1-48和Gobindobhog种子中观察到极低的Rab16A丰度或几乎检测不到的SamDC转录物,而即使在干燥和水吸收的种子中,它们的表达水平也高得多Nonabokra,和较少由ABA诱导。这三个变种中的RAB16A(=脱水蛋白)和SAMDC蛋白表达也与基因表达模式相同。因此,该表达在M-1-48和Gobindobhog中是应激诱导的,而在Nonabokra中是组成性的。我们的研究反映了芳香水稻Gobindobhog种子对外源ABA的分子反应与盐敏感性M-1-48的分子反应相似,仅在胁迫后才表现出较低的耐胁迫蛋白表达。这项工作还证明了种子中基因/蛋白质表达的变化与水稻品种的耐性机制的变化高度相关。

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