Influence of Electroacupuncture Stimulation on Nitric Monoxide Production in Vascular Endothelial Cells in Rats

摘要

Background/Aim: In Chinese medicine, blood stasis termed as 'Oketsu' means 'preceding state' or 'symptomatic of sickness'. Traditional Chinese medicine may improve blood flow by vasodilation or blood clotting inhibition. Although acupuncture influences the blood circulatory system, its underlying mechanisms remain unclear. Herein we evaluated changes in NO, as reflected by changes in NO2-, platelet aggregation, oxidative stress and endocrine responses after acupuncture stimulation in rats. Materials and Methods: Acupuncture stimulation was administered to rats randomly divided into five groups: control, N-G-nitro-L-arginine methyl ester hydrochloride (L-NAME) injection, restraint stress (RS), restraint plus acupuncture stimulation (RA), and restraint plus acupuncture with L-NAME (RLA). Results: Compared to those in the RS group, levels of NO2-, endothelial nitric oxide synthase (NOS) protein and its mRNA significantly increased and those of hydroperoxide and soluble P-selectin significantly decreased in the RA group. Conclusion: Acupuncture stimulation regulates vascular endothelium NOS function and affects vascular resistance and blood characteristics through NO. Additionally, NO produced may modulate excessive reactive oxygen development and blood platelet activation.