Antiapoptotic effect of a novel synthetic peptide from bovine muscle and MPG peptide on H2O2-induced C2C12 cells

摘要

The objective of this study was to evaluate antioxidant and antiapoptotic effects of a novel peptide (T.peptide) isolated from bovine and commercially available MPG peptide. The amino acid sequences of the T.peptide were (Glu-Val-Pro-Glu-Val-His-Glu-Glu-Val). The antioxidant activities of these peptides were determined by measuring the 1,1-diphenyl-2-picrylhydrazyl (DPPHaEuro cent) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+aEuro cent) radical scavenging assays. The in vitro cytotoxicity of T.peptide and MPG peptide was determined against H2O2-induced C2C12 cells. H2O2-induced apoptosis in C2C12 cells were determined by mRNA expression of caspase-3. Moreover, the mRNA expression of tumor necrosis factor alpha (TNF-alpha) and nuclear factor kappa B (NF-kappa B) were assayed by reverse transcription polymerase chain reaction. The findings of the study indicate that the mRNA expression of TNF-alpha and NF-kappa B are significantly (p 0.05) increased in H2O2-induced C2C12 cells, whereas a significant decrease (p 0.05) in these mRNA expressions are observed when pretreated with T.peptide or MPG peptide. Pretreatment with MPG or T.peptides is also found to significantly (p 0.05) decrease the mRNA expression of caspase-3 in H2O2-induced C2C12 cells. The results of the study demonstrate that both T.peptide and MPG peptide could reduce the DPPHaEuro cent and ABTS+aEuro cent radical and inhibit cytotoxicity against H2O2-induced injury, resulting in prevention of free radical generation and subsequent apoptotic cell death, which indicates the potential of bovine meat as a source of antioxidant peptides.