Genetic transformation of duckweed Lemna gibba and Lemna minor

摘要

We developed efficient genetic transformation protocols for two species of duckweed, Lemna gibba (G3) and Lemna minor (8627 and 8744), using Agrobacterium-medialed gene transfer. Partially differentiated nodules were co-cultivated with Agrobacteriumtumefaciens harboring a binary vector containing #bate#-glucuronidase and nptll expression cassettes. Transformed cells were selected and allowed to grow inlo nodules in the presence of kanamycin. Transgenic duckweed fronds were regenerated from selectednodules. We demonstrated that transgenic duckweed could be regenerated within 3 mo. after Agrobacterium-mediated transformation of nodules. Furthermore, we developed a method for transforming L. minor 8627 in 6 wk. These transformation protocols will facilitate genetic engineering of duckweed, ideal plants for bioremediation and large-scale industrial production of biomass and recombinant proteins.