Micropropagation of Uraria picta, a medicinal plant, through axillary bud culture and callus regeneration.

摘要

Micropropagation of U. picta, a leguminous herb, was achieved through axillary bud culture and nodal callus culture. Bud break was best when nodes were cultured on Murashige and Skoog (1962) (MS) medium supplemented with 2.6 鍹 alpha-NAA and 4.4 鍹N6-benzyladenine. Optimum shoot multiplication was observed with adenine sulfate at 2.47 鍹. Competent callus was initiated around the nodal ring of the explant on the basal medium supplemented with cytokinins and auxin (alpha-NAA and benzyladenine), which regenerated into new profusely growing shoots on transferring to 0.13 鍹 benzyladenine. Shoots elongated to 5 nodes with 1.11 鍹 benzyladenine were rooted on half-strength MS basal medium. The rooted plants were successfully established ex vitro with80% survival. About 400 such plants were transferred to the field.