Scale-Up and Optimization studies on Lignin Biodegradation of Lignocellulosic Agro-Waste Jute Stick with P.chrysosporium-An Eco-friendly Pulping Process

摘要

The lignin biodegradation of Jute Stick was studied in the batch suspended growth culture of white-rot-fungi P. chrysosporium. Maximum biodegradation of lignin was obtained after eight days of digestion time. The optimum sugar (glucose) and nitrogen (L-asparagine) content (w/w percent of jute stick) required in a solid-substrate system for lignin biodegradation was studied over a wide range of glucose concentration from 0.5 to 5.0 per cent and nitrogen content from 0.15 to 1.25 per cent. The lignin biodegradation with P. chrysosporium was maximum (66.73%) when the glucose content was 1 per cent and nitrogen concentration was 0.25 per cent after eight days of fermentation. The corresponding pulp had lowest Kappa Number as only 15. For pulp and paper industry, high per cent of lignin degradation is the desirable criteria whereas the Kappa Number should be very low. Glucose content above 1 per cent and nitrogen content above 0.25 per cent did not improve the quality of pulp. The analysis of the effluents after eight days of incubstion showed the average COD and BOD5 values to be the lowest for the optimum MGN Dose II. The colour of the effluent was in the range of 0.240-0.262 (OD at 465 nm).