Determination of Protein Binding Affinities and Investigation into the Antimicrobial Activities of Cu(II), Co(II) and Ni(II) Mixed Ligand Complexes

摘要

The mixed ligand complexes (MLC) of Cu(II), Co(II) and Ni(II) ions with 1,10-phenanthroline (phen), glycine (gly) and tryptophan (trp) are synthesized and characterized. The interactions of these mixed ligand complexes with bovine serum albumin (BSA) have been investigated by fluorescence spectroscopy in combination with UV-VIS spectroscopy under physiological conditions. [Cu(gly)2phen] complex is able to quench to the fluorescence of bovine serum albumin (BSA) indicating the complexation between protein and ligand. The anti-microbial activities of all these complexes have been executed and good anti-microbial activities of the complexes over the ligands have been observed against the gram positive, gram negative bacteria and fungi.