Local immune response at the respiratory tract of calves vaccinated with Pasteurella multocida vaccines.

摘要

The effectiveness of intranasal vaccination of calves with live streptomycin-dependent mutant of P. multocida type B vaccine in comparison with an inactivated vaccine was studied. The live P. multocida vaccine gave a higher mitogenic response of the peripheral blood lymphocytes as measured by lymphocyte blastogenesis assay than inactivated vaccine throughout the experimental period. The haemagglutinating antibodies as measured by passive haemagglutination test (HA) and the IgG as measured by ELISA increased from one day to reach the peak at 15 days, and then decreased until 12 weeks post-vaccination for the live vaccine. However, for the inactivated vaccine, the peak was reached at 8 weeks and then decreased until 12 weeks post-vaccination. Calves with live P. multocida vaccine gave higher and earlier secretory IgA in nasal secretions than the inactivated vaccine. It was concluded that the intranasal administration of live streptomycin-dependent serotype of P. multocida mutant vaccine was efficient. Moreover, it induced local cellular immune response, IgA antibodies and systemic IgG antibodies.