首页> 外文期刊>Annual Report of the Bean Improvement Cooperative >EVALUTION OF COMMON BEAN ELITE LINES DEVELOPED IN BRAZIL FOR ANGULAR LEAF SPOT RESISTANCE
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EVALUTION OF COMMON BEAN ELITE LINES DEVELOPED IN BRAZIL FOR ANGULAR LEAF SPOT RESISTANCE

机译:巴西为角叶斑抗性开发的普通豆线的评估

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摘要

Angular leaf spot (ALS) caused by the fungus Phaeoisariopsis griseola (Sacc.) Ferraris, attacks the common bean (Phaseolus vulgaris L.) in Brazil and in other bean growing regions of the world. We started a recurrent selection bean breeding program tocreate superior varieties with "carioca-type" grains adapted to Brazil. One of the goals of this program is to pyramid disease resistance genes into these lines in order to obtain durable resistance to several pathogens, including P. griseola. As a natural step of the program we evaluated the resistance/susceptibility of 32 "carioca-type" elite lines to seven different pathotypes of P. griseola collected in Brazil (Nietsche et al. 2001). Twelve seeds of each line were sown in the greenhouse and testedwith each of the seven pathotypes. The plants were inoculated with suspensions of P. griseola (2 X 10~4 spores/mL) and incubated for two days in a mist chamber (20°C ± 1 and relative humidity > 95%). Twenty-one days after inoculation the plants were scored visually for the disease symptoms using a 1-9 scale (van Schoonhoven & Pastor-Corrales, 1987). Plants with grades 1 to 3 were considered resistant and with grades 4 to 9, susceptible.
机译:法拉利氏菌(Phaeoisariopsis griseola(Sacc。)Ferraris引起的角叶斑病(ALS)袭击了巴西和世界其他豆类种植地区的普通豆(Phaseolus vulgaris L.)。我们启动了一个轮回选择豆育种计划,以创造出适合巴西的具有“ carioca型”谷物的优良品种。该计划的目标之一是将抗病基因传到这些品系中,以获得对几种病原体(包括灰霉菌)的持久抗性。作为该程序的自然步骤,我们评估了32种“卡里奥卡型”优良品系对巴西收集的7种不同病态的灰霉病的抗性/敏感性(Nietsche等,2001)。将每株系的十二个种子播种在温室中,并用七种致病型进行测试。用灰黄假单胞菌的悬浮液(2×10-4个孢子/ mL)接种植物,并在雾室(20℃±1,相对湿度> 95%)中温育两天。接种21天后,用1-9的量表对植物的病害症状进行视觉评分(van Schoonhoven&Pastor-Corrales,1987)。等级1至3的植物被认为具有抗性,等级4至9的植物易感。

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