ASSESSMENT OF THE CC AND CXC CHEMOKINE FAMILY'S AMOUNTS IN BRON CHOALVEOLAR FLUID AND PERIPHERALBLOOD SAMPLES FROM PATIENTS WITH PULMONARY TUBERCULOSIS IN MASHHAD PERSIAN DATE 2013 TO 2014 IN COMPARISON TO HEALTHY PEOPLE

摘要

Pulmonary tuberculosis is one of the most common infectious diseases; the causative agent of the disease is acid fast bacilli called Mycobacterium tuberculosis. Approximately one-third of the world's population is infected with the bacteria. This disease despite extensive control programs in the past two decades is still one of the most dangerous infectious diseases. As it is estimated that around the world people develop TB disease 15 patient every 1 minutes and 6 die from this disease. For prevention of TB, early detection of the disease may be one of the most important strategies. This cross-sectional study was conducted on a group of 150 patients with pulmonary tuberculosis, were involved. Peripheral blood and BAL was performed for patients as well as controls carried out in coordination with the Mashhad and 150 healthy individuals were collected, and blood was obtained. Demographic data were collected through clinical history and methods, and were matched for age and sex. The chemokine familyof CC and CXC in peripheral blood samples and bronchoalveolar lavage samples were measured and the results , along with demographic information by SPSS software and statistical method T-Test and Mann - Whitney were analysised. One hundred and fifty patients were involved in the scheme. They were matched for age and sex . The serum level mean were measured every 3 CC chemokines family's in peripheral blood and BAL in patients with pulmonary tuberculosis had increased significantly and statistical differences were significant (P<0.0001). The serum level mean of CXC4 chemokine family in peripheral blood and BAL in patients with pulmonary tuberculosis in peripheral blood than the control group also showed significant difference except one (The comparison of serum level mean of CXCL9 (Mig) in peripheral blood between patients and controls was not significant (P = 0.092). Our study showed that all CC and CXC chemokine families in the study of BAL (bronchoalveolar lavage) sample in pulmonary tuberculosis patients was significantly associated with the disease, and suggests CCL11 as a new chemokine associated with pulmonary tuberculosis in bronchoalveolar fluid that could serve as a diagnostic marker from alveolar fluid to be used as well all the items in theperipheral blood of patients with pulmonary tuberculosis in chemokine significantly related to disease and are discussed as tuberculosis diagnostic markers in blood, except CXCL9 (Mig) that local nature of this chemokine probably is production in bronchial epithelial cells, thus making it suggestive tuberculosis marker only in BAL.