Bio-Analytical Liquid Chromatographic Method for the Determination of Linezolid in Plasma

摘要

An isocratic selective and sensitive high performance liquid chromatographic method for the estimation of linezolid in human plasma by precipitation of plasma proteins with 5 % perchloric acid, after precipitation the supernatant was separated and injecting 10 uL of sample volume. The chromatography separation was achieved on a Hypersil BDS C_(18) (4.6 ID x 250 mm, 5 μ particle size) column using a mixture of 20 Mm potassium dihydrogen phosphate buffer (pH 3.5 ± 0.1) and acetonitrile in a ratio of 85:15 (v/v) as the mobile phase. The column elutes were monitored at 252 nm. The total chromatographic run time was 12.5 min and the elution of linezolid and zidovudine (IS) retention times 10.5 and 5.2 respectively. The method was found to be linear in the concentration range of 50.0 to 20059.9 ng/mL. The method was successfully applied to in vitro pharmacokinetic-bioequivalence studies.