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Study on the Antibacterial and Bactericidal Effects of Ailanthus altissima Leaves Extract

机译:臭椿叶提取物的抑菌和杀菌作用研究

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In present studies, the antibacterial and bactericidal effection of the A. altissima leaves extract are reported. Three different concentrations of A. altissima leaves extract were tested for the activities of antibacterial and bactericidal on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The inhibition zone diameter of the three bacteria was tested using the method of filter paper diffusion. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested by the double dilution method. Three different concentrations of A. altissima leaves extract all have antimicrobial effect. Therefore, the inhibitory effect is more evident at higher concentration of the drug. The same concentration of Ailanthus altissima leaves extract on different amounts of bacterial suspensions have inhibitory effect, the greater the amount of bacterial suspension, the bigger inhibition zone diameter, the inhibitory effect is more evident. The minimum inhibitory concentrations of A. altissima leaf extract against S. aureus, P. aeruginosa, E. coli were 35.0, 8.8 and 70.0 mg/mL, respectively. The inhibitory effect of P. aeruginosa is the strongest. The minimum bactericidal concentration of A. altissima leaf extract against S. aureus, P. aeruginosa, E. coli were 35.0,17.5 and 70.0 mg/mL, respectively. Therefore, the bactericidal effect on P. aeruginosa is the strongest.
机译:在本研究中,报道了A. altissima叶子提取物的抗菌和杀菌作用。测试了三种不同浓度的拟南芥叶提取物对大肠杆菌,金黄色葡萄球菌和铜绿假单胞菌的抗菌和杀菌活性。使用滤纸扩散的方法测试了三种细菌的抑制区直径。通过双重稀释法测试最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。三种不同浓度的拟南芥叶提取物均具有抗菌作用。因此,在药物的较高浓度下抑制作用更加明显。相同浓度的臭椿叶提取物对不同量的细菌悬液均有抑制作用,细菌悬液量越大,抑制带直径越大,抑制作用越明显。拟南芥叶提取物对金黄色葡萄球菌,铜绿假单胞菌和大肠杆菌的最低抑制浓度分别为35.0、8.8和70.0 mg / mL。铜绿假单胞菌的抑制作用最强。 altissima叶提取物对金黄色葡萄球菌,铜绿假单胞菌和大肠杆菌的最低杀菌浓度分别为35.0、17.5和70.0 mg / mL。因此,对铜绿假单胞菌的杀菌作用最强。

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